BRSK2 Kinase Assay Service - Reaction Biology

Activity-based BRSK2 kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

BRSK2

Enzyme

Human BRSK2

HGNC

BRSK2

Synonym

C11orf7, FLJ41362, HUSSY-12, PEN11B, SAD1, STK29

Substrates

Peptide substrate, [KKLNRTLSFAEPG], 20 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

Q8IWQ3 (http://www.uniprot.org/uniprot/Q8IWQ3)

Screening Location

Malvern, USA

Reference compound IC50 for BRSK2