BTK (Y485F) Kinase Assay Service

Activity-based BTK (Y485F) kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

BTK (Y485F)

Enzyme

Human BTK (Y485F)

HGNC

BTK (Y485F)

Synonym

Tyrosine-protein kinase BTK, Bruton tyrosine kinase, Agammaglobulinaemia tyrosine kinase, ATK, B cell progenitor kinase, BPK, AGMX1, AT, PSCTK1

Substrates

Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

Q06187 (http://www.uniprot.org/uniprot/Q06187)

Screening Location

Malvern, USA

Reference compound IC50 for BTK (Y485F)