c-KIT (V559D/V654A) Kinase Assay Service

Activity-based c-KIT (V559D/V654A) kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

c-KIT (V559D/V654A)

Enzyme

Human c-KIT (V559D/V654A)

HGNC

KIT (V559D/V654A)

Synonym

C-Kit, PBT, CD117, SCFR,

Substrates

Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

P10721 (http://www.uniprot.org/uniprot/P10721)

Screening Location

Malvern, USA

Reference compound IC50 for c-KIT (V559D/V654A)