Activity-based c-KIT (V654A) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
c-Kit (V654A)
Enzyme
Human c-Kit (V654A)
HGNC
KIT (V654A)
Synonym
C-Kit, PBT, CD117, SCFR,
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.1 mg/ml
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Scientific Information
P10721 (http://www.uniprot.org/uniprot/P10721)
Screening Location
Malvern, USA
Reference compound IC50 for c-Kit (V654A)