Activity-based c-MET (D1228H) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
c-MET (D1228H)
Enzyme
Human c-MET (D1228H)
HGNC
MET (D1228H)
Synonym
Hepatocyte growth factor receptor, HGF receptor, Scatter factor receptor, SF receptor, HGF/SF receptor, Met proto-oncogene tyrosine kinase, c-Met, MET
Scientific Information
P08581 (http://www.uniprot.org/uniprot/P08581)
Assay Properties
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/mL
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 27 |
| PP2 | >100,000 |
| AG1478 | 17,000 |
| EGFR/Erb Inhibitor | >100,000 |
| D-64406 | 9,300 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for c-MET (D1228H)
