c-Src Kinase Assay Service - Reaction Biology

Activity-based c-Src kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

c-SRC

Enzyme

Human c-SRC

HGNC

SRC

Synonym

ASV, SRC1, c-SRC, p60-Src

Substrates

Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

P12931 (http://www.uniprot.org/uniprot/P12931)

Screening Location

Malvern, USA

Reference compound IC50 for c-Src