Activity-based c-SRC (Y530F) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
c-SRC (Y530F)
Enzyme
Human c-SRC (Y530F)
HGNC
SRC (Y530F)
Synonym
Proto-oncogene tyrosine-protein kinase Src, pp60c-src, ASV, SRC1, c-SRC, p60-Src
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Scientific Information
P12931 (http://www.uniprot.org/uniprot/P12931)
Screening Location
Malvern, USA
Reference compound IC50 for c-SRC (Y530F)
