CAMK1d Kinase Assay Service - Reaction Biology

Activity-based CAMK1d kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

CAMK1d

Enzyme

Human CAMK1d

HGNC

CAMK1D

Synonym

CKLiK, CaM-K1, CaMKID

Substrates

Peptide substrate, [KKALRRQETVDAL], 20 µM
Ca²⁺-Calmodulin, 1 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

Q8IU85 (http://www.uniprot.org/uniprot/Q8IU85)

Screening Location

Malvern, USA

Reference compound IC50 for CAMK1d