CAMK2b Kinase Assay Service - Reaction Biology

Activity-based CAMK2b kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

CAMK2b

Enzyme

Human CAMK2b

HGNC

CAMK2B

Synonym

CAM2, CAMK2, CAMKB, MGC29528

Substrates

Peptide substrate, [KKALRRQETVDAL], 20 µM
Ca²⁺-Calmodulin, 1 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

Q13554 (http://www.uniprot.org/uniprot/Q13554)

Screening Location

Malvern, USA

Reference compound IC50 for CAMK2b