CAMK2d Kinase Assay Service - Reaction Biology

Activity-based CAMK2d kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

CAMK2d

Enzyme

Human CAMK2d

HGNC

CAMK2D

Synonym

CAMKD, MGC44911, CaMKII delta

Substrates

Peptide substrate, [KKLNRTLSFAEPG], 20 µM
Ca²⁺-Calmodulin, 1 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

Q13557 (http://www.uniprot.org/uniprot/Q13557)

Screening Location

Malvern, USA

Reference compound IC50 for CAMK2d