Activity-based CAMK2g kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
CAMKIIγ
Enzyme
Human CAMKIIγ
HGNC
CAMK2G
Synonym
CAMK, CAMK-II, CAMKG, MGC26678
Substrates
Peptide substrate, [KKLNRTLSFAEPG], 20 µM
Ca2+-Calmodulin, 1 µM
ATP 10 µM
Ca2+-Calmodulin, 1 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Scientific Information
Q13555 (http://www.uniprot.org/uniprot/Q13555)
Screening Location
Malvern, USA
Reference compound IC50 for CAMKIIγ
