Activity-based CAMKK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
CAMKK1
Enzyme
Human CAMKK1
HGNC
CAMKK1
Synonym
CAMKKA, DKFZp761M0423, MGC34095
Substrates
MBP (Myelin Basic Protein), 10 µM
Ca2+-Calmodulin, 1 µM
ATP 10 µM
Ca2+-Calmodulin, 1 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Scientific Information
Q8N5S9 (http://www.uniprot.org/uniprot/Q8N5S9)
Screening Location
Malvern, USA
Reference compound IC50 for CAMKK1
