Cav1.2 (L-type voltage-gated calcium channel) Manual Patch Clamp InVEST44 Assay for compound screening and profiling via luminescence recordings.
Target Overview
CAT#
Cav1.2 MPC InVEST44
GPCR Target
Human Cav1.2
Cell Line
CHO-K1 cells overexpressing human Cav1.2
Scientific Information
CAC1C (https://www.uniprot.org/uniprotkb/Q13936/entry) and others
Assay Properties
Protocol
Individual cells expressing Cav1.2 studied using whole-cell manual patch electrophysiology; compounds bath applied using constant gravity perfusion for 2-3 min or until steady state current reached
Readout
Maximum negative current (pA) elicited by a +10 mV step from -80 mV
Reference Compound IC50
| Compound IC50 (nM) |
|---|
| Nifedipine (53) |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website In Vitro Safety Pharmacology Profiling
Reference compound inhibition of Cav1.2
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