Cannabinoid receptor 1 (CB1) Biochemical Binding Assay for compound screening and profiling via competitive radioligand binding.
Target Overview
CAT#
CB1 Binding
GPCR Target
Human CB1
Source
Membrane preparation from HEK-293 cells stably transfected with human CB1 receptor
Scientific Information
CNR1 (https://www.uniprot.org/uniprotkb/P21554/entry)
Assay Properties
Protocol
Radioligand filter binding to CB1 membrane preparation ± cold competing ligand; radiometric measurement on MicroBeta scintillation counter
Readout
CPM representing 3H-disintegration detected using MicroScint-20
Reference Compound IC50
| Compound IC50 (nM) | Radioligand (nM) |
|---|---|
| CP55,940 (6.5) | CP55,940 (1) |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website In Vitro Safety Pharmacology Profiling
Reference compound binding data for CB1
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