Activity-based CDC42BPB kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
CDC42BPB
Enzyme
Human CDC42BPB, wt
HGNC
CDC42BPB
Manning ID
MRCKb
Synonym
MRCK-beta, MRCKb, CDC42BPB, MRCKb, MRCKb/CDC42BPB
UniProt
Q9Y5S2 (MRCKB_HUMAN)
Kinase Type
Protein kinase
Kinase Group
AGC
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RB-S6P, ATP
Reaction
RB-S6P + 33P-ATP = 33P-RB-S6P + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 7.5 |
| Sorafenib | 9300 |
| Sunitinib | 61000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | CDC42BPB |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – CDC42BPB (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 0382-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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