Activity-based CDC7/DBF4 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
CDC7/DBF4
Enzyme
Human CDC7/DBF4, wt
HGNC
CDC7-DBF4
Manning ID
CDC7
Synonym
CDC7L1, HsCDC7, Hsk1, huCDC7, CDC7, HsCdc7, huCdc7, CDC7/DBF4
UniProt
O00311 (CDC7_HUMAN)
Kinase Type
Protein kinase
Kinase Group
Other
Assay Properties
Assay Format
33PanQinaseTM
Substrates
Histon H1, ATP
Reaction
Histon H1 + 33P-ATP = 33P-Histon H1 + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 170 |
| Sorafenib | 10000 |
| Sunitinb | 100000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | CDC7/DBF4 |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – CDC7/DBF4 (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 1513-1516-1 |
| Web Shop | Reaction Biology Online Shop |
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