CDC7/DBF4 Kinase Assay Service

Activity-based CDC7/DBF4 kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

CDC7/DBF4

Enzyme

Human CDC7/DBF4

HGNC

CDC7/DBF4

Synonym

CDC7L1, HsCdc7, HsCDC7, Hsk1, huCdc7

Substrates

Peptide substrate, [KTFCGTPEYLAPEVRREPRILSEEEQEMFRDFDYIADWC], 20 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

CDC7: O00311 (http://www.uniprot.org/uniprot/O00311)DBF4: Q9UBU7 (http://www.uniprot.org/uniprot/Q9UBU7)

Screening Location

Malvern, USA

Reference compound IC50 for CDC7/DBF4