CDK10+Cyclin L2 NanoBRET Kinase Assay

CDK10+Cyclin L2 NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.
Target Overview

Target

CDK10+Cyclin L2

HGNC Symbol

CDK10/CCNL2 complex

Synonyms

PISSLRE

Cell Line

HEK293 human epithelial cell line from ATCC

Assay Properties

Assay Format

384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay

Assay Protocol

HEK293 cells transiently co-expressing NanoLuc- CDK10 Fusion Vector and Cyclin L2 Vector were seeded into the wells of 384-well plates. The cells were pretreated with the NanoBRET Tracer K-10 and then treated with reference compound AT7519 for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism 4 program based on a sigmoidal dose response equation.

Readout

Binding of compound to target kinase

Reference Compound IC50s
Compounds IC50 (M)
AT7519 3.589e-006

Screening Location

Malvern, USA

Reference compound IC50 for CDK10+Cyclin L2