CDK11B/Cyclin K Kinase Assay Service

Activity-based CDK11B/Cyclin K kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.

Target Overview
CAT#

CDK11B/Cyclin K

Enzyme

Human CDK11B/Cyclin K

HGNC

CDK11B/CCNK

Synonym

CDK11B: Cyclin-dependent kinase 11B, CDC2L1, PITSLREA, PK58
Cyclin K: CPR4, CCNK

Scientific Information

CDK11B: P21127 (http://www.uniprot.org/uniprot/P21127)
Cyclin K: O75909 (http://www.uniprot.org/uniprot/O75909)

Assay Properties
Substrates

Protein substrate, SUPT5H, 5 µM
ATP, 10 µM

Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine >100,000
Ro 31-8220 >100,000
GW 5074 >100,000
AT-7519 28
OTS964 310
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays

Reference compound IC50 for CDK11B/cyclin K