CDK12/cyclin K Kinase Assay Service

Activity-based CDK12/cyclin K kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.

Target Overview
CAT#

CDK12/Cyclin K

Enzyme

Human CDK12/Cyclin K

HGNC

CDK12/CCNK

Synonym

CRKRS, CRK7

Substrates

Protein substrate, Pol2-CTD, 5 µM
ATP 10 µM

Assay Properties
Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 120
Ro 31-8220 20
GW 5074 28,000
H-89 1,100
THZ531 45
Scientific Information

CDK12: Q9NYV4 (http://www.uniprot.org/uniprot/Q9NYV4)

Cyclin K: O75909 (http://www.uniprot.org/uniprot/O75909)

Screening Location

Malvern, USA

Reference compound IC50 for CDK12/cyclin K