Activity-based CDK12/cyclin K kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
CDK12/Cyclin K
Enzyme
Human CDK12/Cyclin K
HGNC
CDK12/CCNK
Synonym
CRKRS, CRK7
Substrates
Protein substrate, Pol2-CTD, 5 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Compounds | IC50 (nM) |
---|---|
Staurosporine | 120 |
Ro 31-8220 | 20 |
GW 5074 | 28,000 |
H-89 | 1,100 |
THZ531 | 45 |
Scientific Information
Screening Location
Malvern, USA
Reference compound IC50 for CDK12/cyclin K