Activity-based CDK12 R722C/CycK kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
CDK12 R722C/CycK
Enzyme
Human CDK12 R722C/CycK, mutant
HGNC
CDK12 (R722C)-CCNK
Manning ID
CDK10
Synonym
CDK12, CRKRS, CRK7, CDK12 R722C/CycK, CDK12 (R722C)/cyclin K
UniProt
Q9NYV4 (CDK12_HUMAN)
Kinase Type
Protein kinase
Kinase Group
CMGC
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RBER-IRStide, ATP
Reaction
RBER-IRStide + 33P-ATP = 33P-RBER-IRStide + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 100 |
| Sorafenib | 10000 |
| Sunitinib | 30000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | CDK12 R722C/CycK |
| 355 wt Profiler | No |
| 355 IC50 wt Profiler | No |
| Mutant Profiler Addon | Yes |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – CDK12 R722C/CycK (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 1488-1484-1 |
| Web Shop | Reaction Biology Online Shop |
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