CDK12 (R722C)/cyclin K Kinase Assay Service

Activity-based CDK12 (R722C)/cyclin K kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

CDK12 (R722C)/Cyclin K

Enzyme

Human CDK12 (R722C)/Cyclin K

HGNC

CDK12 (R722C)/CCNK

Synonym

CRKRS, CRK7

Substrates

Protein substrate, Pol2-CTD, 5 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

CDK12: Q9NYV4 (http://www.uniprot.org/uniprot/Q9NYV4)Cyclin K: O75909 (http://www.uniprot.org/uniprot/O75909)

Screening Location

Malvern, USA

Reference compound IC50 for CDK12 (R722C)/cyclin K