Activity-based CDK15/CycA2 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
CDK15/CycA2
Enzyme
Human CDK15/CycA2, wt
HGNC
CDK15-CCNA2
Manning ID
PFTAIRE2
Synonym
PFTAIRE2, ALS2CR7, PFTK2, CDK15
UniProt
Q96Q40 (CDK15_HUMAN)
Kinase Type
Protein kinase
Kinase Group
CMGC
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RBER-NTRK3tide, ATP
Reaction
RBER-NTRK3tide + 33P-ATP = 33P-RBER-NTRK3tide + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 18.5 |
| Sorafenib | 10000 |
| Sunitinib | 63000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | CDK15/CycA2 |
| 355 wt Profiler | No |
| 355 IC50 wt Profiler | No |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | IC50 CDK Profiler |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – CDK15/CycA2 (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 1889-0054-1 |
| Web Shop | Reaction Biology Online Shop |
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