CDK15/Cyclin A2 Kinase Assay Service

Activity-based CDK15/Cyclin A2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.

Target Overview
CAT#

CDK15/Cyclin A2

Enzyme

Human CDK15/Cyclin A2

HGNC

CDK15/CCNA2

Synonym

CDK15: Cyclin-dependent kinase 15, Cell division protein kinase 15, PFTK2
Cyclin A2: CCNA2

Scientific Information

CDK15: Q96Q40 (http://www.uniprot.org/uniprot/Q96Q40)
Cyclin A2: P20248 (http://www.uniprot.org/uniprot/P20248)

Assay Properties
Substrates

Protein substrate, RB Protein, 5 µM
ATP, 10 µM

Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 0.8
Ro 31-8220 5.8
GW 5074 14,000
H-89 3,900
Rottlerin 2,200
Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Kinase Assays.