CDK15/Cyclin B1 Kinase Assay Service

Activity-based CDK15/Cyclin B1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.

Target Overview
CAT#

CDK15/Cyclin B1

Enzyme

Human CDK15/Cyclin B1

HGNC

CDK15/CCNB1

Synonym

CDK15: Cyclin-dependent kinase 15, Cell division protein kinase 15, PFTK2
Cyclin B1: CCNB1

Scientific Information

CDK15: Q96Q40 (http://www.uniprot.org/uniprot/Q96Q40)
Cyclin B1: P14635 (http://www.uniprot.org/uniprot/P14635)

Assay Properties
Substrates

Protein substrate, RB Protein, 5 µM
ATP, 10 µM

Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 1.9
Ro 31-8220 2.4
GW5074 8,400
H-89 15,000
Abemaciclib 43
Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Kinase Assays.

Reference compound IC50 for CDK15/Cyclin B1