Activity-based CDK17/p35NCK kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
CDK17/p35NCK
Enzyme
Human CDK17/p35NCK, wt
HGNC
CDK17-CDK5R1
Manning ID
PCTAIRE2
Synonym
PCTAIRE2, PCTK2, CDK17, CDK17/p35NCK
UniProt
Q00537 (CDK17_HUMAN)
Kinase Type
Protein kinase
Kinase Group
CMGC
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RBER-CDC25tide, ATP
Reaction
RBER-CDC25tide + 33P-ATP = 33P-RBER-CDC25tide + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 320 |
| Sorafenib | 10000 |
| Sunitinib | 9100 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | CDK17/p35NCK |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | IC50 CDK Profiler |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – CDK17/p35NCK (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 1526-0355-1 |
| Web Shop | Reaction Biology Online Shop |
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