CDK1/cyclin A Kinase Assay Service

Activity-based CDK1/cyclin A kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.

Target Overview
CAT#

CDK1/cyclin A

Enzyme

Human CDK1/cyclin A

HGNC

CDK1/CCNA2

Synonym

CDC2, p34

Substrates

RB Protein, 3 µM
ATP 10 µM

Assay Properties
Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 3.5
Ro 31-8220 45
H-89 16,000
GW5074 81,000
Rottlerin >100,000
Scientific Information

CDK1: P06493 (http://www.uniprot.org/uniprot/P06493)
Cyclin A: P20248 (http://www.uniprot.org/uniprot/P20248)

Screening Location

Malvern, USA