CDK1/cyclin E Kinase Assay Service

Activity-based CDK1/cyclin E kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

CDK1/Cyclin E

Enzyme

Human CDK1/Cyclin E

HGNC

CDK1/CCNE1

Synonym

CDC2, p34

Substrates

RB protein, 1 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

CDK1: P06493 (http://www.uniprot.org/uniprot/P06493)Cyclin E: P24864 (http://www.uniprot.org/uniprot/P24864)

Screening Location

Malvern, USA

Reference compound IC50 for CDK1/cyclin E