Activity-based CDK2/CycE1 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
CDK2/CycE1
Enzyme
Human CDK2/CycE1, wt
HGNC
CDK2-CCNE1
Manning ID
CDK2
Synonym
p33(CDK2), CDK2, p33, CDK2/CycE1, CDK2/cyclin E
UniProt
P24941 (CDK2_HUMAN)
Kinase Type
Protein kinase
Kinase Group
CMGC
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RBER-CHKtide, ATP
Reaction
RBER-CHKtide + 33P-ATP = 33P-RBER-CHKtide + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 11 |
| Sorafenib | 10000 |
| Sunitinb | 15000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | CDK2/CycE1 |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | IC50 CDK Profiler |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – CDK2/CycE1 (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 0050-0055-1 |
| Web Shop | Reaction Biology Online Shop |
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