CDK2/cyclin A Kinase Assay Service

Activity-based CDK2/cyclin A kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.

Target Overview
CAT#

CDK2/Cyclin A2

Enzyme

Human CDK2/Cyclin A2

HGNC

CDK2/CCNA2

Synonym

p33

Substrates

RB Protein, 3 µM
ATP 10 µM

Assay Properties
Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 1.0
Ro 31-8220 4.9
GW 5074 >100,000
H-89 3,600
Rottlerin >100,000
Scientific Information

CDK2: P24941 (http://www.uniprot.org/uniprot/P24941)Cyclin A2: P20248 (http://www.uniprot.org/uniprot/P20248)

Screening Location

Malvern, USA