CDK2/cyclin E2 Kinase Assay Service

Activity-based CDK2/cyclin E2 kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

CDK2/cyclin E2

Enzyme

Human CDK2/cyclin E2

HGNC

CDK2/CCNE2

Synonym

p33

Substrates

RB protein, 3 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

CDK2: P24941 (http://www.uniprot.org/uniprot/P24941)Cyclin E2: O96020 (http://www.uniprot.org/uniprot/O96020)

Screening Location

Malvern, USA

Reference compound IC50 for CDK2/cyclin E2