CDK3/Cyclin C Kinase Assay Service

Activity-based CDK3/Cyclin C kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.

Target Overview
CAT#

CDK3/Cyclin C

Enzyme

Human CDK3/Cyclin C

HGNC

CDK3/CCNC

Synonym

CDK3: Cell division protein kinase 3, Cyclin-dependent kinase 3
Cyclin C: CCNC

Scientific Information

CDK3: Q00526 (http://www.uniprot.org/uniprot/Q00526)
Cyclin C: P24863 (http://www.uniprot.org/uniprot/P24864)

Assay Properties
Substrates

Peptide substrate, [KKKVSRSGLYRSPSMPENLNRPR], 20 µM
ATP, 10 µM

Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 0.5
Ro 31-8220 52
GW5074 550
H-89 2,700
Abemaciclib >100,000
Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Kinase Assays

Reference compound IC50 for CDK3/Cyclin C