Activity-based CDK4/CycD1 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
CDK4/CycD1
Enzyme
Human CDK4/CycD1, wt
HGNC
CDK4-CCND1
Manning ID
CDK4
Synonym
CMM3, PSK-J3, MGC14458, CDK4, CDK4/CycD1, CDK4/cyclin D1
UniProt
P11802 (CDK4_HUMAN)
Kinase Type
Protein kinase
Kinase Group
CMGC
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RBER-CHKtide, ATP
Reaction
RBER-CHKtide + 33P-ATP = 33P-RBER-CHKtide + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 46 |
| Sorafenib | 10000 |
| Sunitinb | 10000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | CDK4/CycD1 |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | IC50 CDK Profiler |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – CDK4/CycD1 (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 0142-0143-1 |
| Web Shop | Reaction Biology Online Shop |
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