CDK4/cyclin D1 Kinase Assay Service

Activity-based CDK4/cyclin D1 kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

CDK4/Cyclin D1

Enzyme

Human CDK4/Cyclin D1

HGNC

CDK4/CCND1

Synonym

CMM3, PSK-J3

Substrates

RB protein, 3 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

CDK4: P11802 (http://www.uniprot.org/uniprot/P11802)Cyclin D1: P24385 (http://www.uniprot.org/uniprot/P24385)

Screening Location

Malvern, USA

Reference compound IC50 for CDK4/Cyclin D1