Activity-based CDK5/p25NCK kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
CDK5/p25NCK
Enzyme
Human CDK5/p25NCK, wt
HGNC
CDK5-CDK5R1 (p25)
Manning ID
CDK5
Synonym
PSSALRE, CDK5, CDK5/p25NCK, CDK5/p25
UniProt
Q00535 (CDK5_HUMAN)
Kinase Type
Protein kinase
Kinase Group
CMGC
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RBER-CHKtide, ATP
Reaction
RBER-CHKtide + 33P-ATP = 33P-RBER-CHKtide + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 19 |
| Sorafenib | 10000 |
| Sunitinb | 6000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | CDK5/p25NCK |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | IC50 CDK Profiler |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – CDK5/p25NCK (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 0356-0389-1 |
| Web Shop | Reaction Biology Online Shop |
PDF