CDK5/p25 Kinase Assay Service

Activity-based CDK5/p25 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.

Target Overview
CAT#

CDK5/p25

Enzyme

Human CDK5/p25

HGNC

CDK5/CDK5R1

Synonym

PSSALRE

Substrates

RB Protein, 3 µM
ATP 10 µM

Assay Properties
Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 3.1
Ro 31-8220 13
GW 5074 >100,000
H-89 13,000
Rottlerin >100,000
Scientific Information

CDK5: Q00535 (http://www.uniprot.org/uniprot/Q00535)P25: Q15078 (http://www.uniprot.org/uniprot/Q15078)

Screening Location

Malvern, USA

Reference compound IC50 for CDK5/p25