Activity-based CDK6/CycD1 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
CDK6/CycD1
Enzyme
Human CDK6/CycD1, wt
HGNC
CDK6-CCND1
Manning ID
CDK6
Synonym
PLSTIRE, MGC59692, CDK6, CDK6/CycD1, CDK6/cyclin D1
UniProt
Q00534 (CDK6_HUMAN)
Kinase Type
Protein kinase
Kinase Group
CMGC
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RBER-CHKtide, ATP
Reaction
RBER-CHKtide + 33P-ATP = 33P-RBER-CHKtide + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 8.6 |
| Sorafenib | 10000 |
| Sunitinib | 2800 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | CDK6/CycD1 |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | IC50 CDK Profiler |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – CDK6/CycD1 (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 0051-0143-2 |
| Web Shop | Reaction Biology Online Shop |
PDF