CDK6/cyclin D1 Kinase Assay Service

Activity-based CDK6/cyclin D1 kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

CDK6/Cyclin D1

Enzyme

Human CDK6/Cyclin D1

HGNC

CDK6/CCND1

Synonym

PLSTIRE

Substrates

RB protein, 3 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

CDK6: Q00534 (http://www.uniprot.org/uniprot/Q00534)Cyclin D1: P24385 (http://www.uniprot.org/uniprot/P24385)

Screening Location

Malvern, USA

Reference compound IC50 for CDK6/cyclin D1