CDK6/cyclin D2 Kinase Assay Service

Activity-based CDK6/cyclin D2 kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

CDK6/cyclin D2

Enzyme

Human CDK6/Cyclin D2

HGNC

CDK6-CCND2

Synonym

CDK6: Cell division protein kinase 6, Serine/threonine-protein kinase PLSTIRE
Cyclin D2: G1/S-specific cyclin-D2, CCND2

Substrates

RB protein, 3 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

CDK6: Q00534 (http://www.uniprot.org/uniprot/Q00534)
Cyclin D2: P30279 (http://www.uniprot.org/uniprot/P30279)

Screening Location

Malvern, USA

Reference compound IC50 for CDK6/cyclin D2