CDK6/cyclin D3 Kinase Assay Service

Activity-based CDK6/cyclin D3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
CDK6/Cyclin D3
Enzyme
Human CDK6/Cyclin D3
HGNC
CDK6/CCND3
Synonym
PLSTIRE
Substrates
RB protein, 1 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Scientific Information
CDK6: Q00534 (http://www.uniprot.org/uniprot/Q00534)Cyclin D3: P30281 (http://www.uniprot.org/uniprot/P30281)
Screening Location
Malvern, USA
Reference compound IC50 for CDK6/cyclin D3