CDK7/Cyclin H/MNAT1 Kinase Assay Service

Activity-based CDK7/Cyclin H/MNAT1 kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

CDK7/Cyclin H/MNAT1

Enzyme

Human CDK7/Cyclin H/MNAT1

HGNC

CDK7/CCNDH

Synonym

CDK7: Cell division protein kinase 7, CDK-activating kinase, CAK, CAK1, P39 Mo15, STK1, TFIIH basal transcription factor complex kinase subunit, 39 kDa protein kinase, MO15
Cyclin H: MO15-associated protein, p37, p34, CCNH
MNAT1: CDK-activating kinase assembly factor MAT1, RING finger protein MAT1, p36, p35,Menage a trios, CDK7/cyclin H assembly factor, Cyclin G1-interacting protein, RING finger protein 66, CAP35, MAT1, RNF66

Scientific Information

CDK7: P50613 (http://www.uniprot.org/uniprot/P50613)
Cyclin H: P51946 (http://www.uniprot.org/uniprot/P51946)
MNAT1: P51948 (http://www.uniprot.org/uniprot/P51948)

Assay Properties

Substrates

Protein substrate, Pol2-CTD, 5 µM
ATP, 10 µM

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Compounds	IC50 (nM)
Staurosporine	13
Ro 31-8220	10.9
GW 5074	8,000
H-89	14,900
Rottlerin	21,700

Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Kinase Assays

Reference compound IC50 for CDK7/Cyclin H/MNAT1