Activity-based CDK8/CycC kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
CDK8/CycC
Enzyme
Human CDK8/CycC, wt
HGNC
CDK8-CCNC
Manning ID
CDK8
Synonym
K35, CDK8, CDK8/CycC, CDK8/cyclin C
UniProt
P49336 (CDK8_HUMAN)
Kinase Type
Protein kinase
Kinase Group
CMGC
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RBER-CHKtide, ATP
Reaction
RBER-CHKtide + 33P-ATP = 33P-RBER-CHKtide + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 30 |
| Sorafenib | 110 |
| Sunitinib | 100000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | CDK8/CycC |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | IC50 CDK Profiler |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – CDK8/CycC (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 0376-0390-1 |
| Web Shop | Reaction Biology Online Shop |
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