Activity-based CDK9/CycK kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
CDK9/CycK
Enzyme
Human CDK9/CycK, wt
HGNC
CDK9-CCNK
Manning ID
CDK9
Synonym
C-2k, CDC2L4K35, PITALRE, TAK, CDK9, CDC2L4, CDC2-related kinase, CDK9/CycK, CDK9/cyclin K
UniProt
P50750 (CDK9_HUMAN)
Kinase Type
Protein kinase
Kinase Group
CMGC
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RBER-CHKtide, ATP
Reaction
RBER-CHKtide + 33P-ATP = 33P-RBER-CHKtide + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 22 |
| Sorafenib | 10000 |
| Sunitinib | 8900 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | CDK9/CycK |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | IC50 CDK Profiler |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – CDK9/CycK (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 0371-1484-1 |
| Web Shop | Reaction Biology Online Shop |
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