CDK9/Cyclin K Kinase Assay Service

Activity-based CDK9/Cyclin K kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
CDK9/Cyclin K
Enzyme
Human CDK9/Cyclin K
HGNC
CDK9/CCNK
Synonym
Cell division protein kinase 9, Cyclin-dependent kinase 9, Serine/threonine-protein kinase PITALRE, C-2K, Cell division cycle 2-like protein kinase 4, CDC2L4
Cyclin K: Cyclin-K, CCNK, CPR4
Scientific Information
CDK9: P50750 (http://www.uniprot.org/uniprot/P50750)
Cyclin K: O75909 (http://www.uniprot.org/uniprot/O75909)
Assay Properties
Substrates
Protein substrate, Pol2-CTD, 5 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 5.1
Ro 31-8220 2.1
GW 5074 9,900
H-89 5,500
Rottlerin >100,000
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for CDK9/cyclin K
Reference compound IC50 for CDK9/cyclin K
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