Central BDZ (GABAA benzodiazepine binding site) Biochemical Binding Assay for compound screening and profiling via competitive radioligand binding.
Target Overview
CAT#
Central BDZ Binding
GPCR Target
Rat Central BDZ
Source
Membrane preparation from homogenized whole rat brain preparation containing heterogeneous rat GABAA receptors
Scientific Information
GBRA1 (https://www.uniprot.org/uniprotkb/P62813/entry) and others
Assay Properties
Protocol
Radioligand filter binding to GABAA membrane preparation ± cold competing ligand; radiometric measurement on MicroBeta scintillation counter
Readout
CPM representing 3H-disintegration detected using MicroScint-20
Reference Compound IC50
| Compound IC50 (nM) | Radioligand (nM) |
|---|---|
| Flumazenil (8.1) | Ro151788 (10) |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website In Vitro Safety Pharmacology Profiling
Reference compound binding for Central BDZ
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