CHK1 Kinase Assay Service

Activity-based CHK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
CHK1

Enzyme
Human CHK1

HGNC
CHEK1

Synonym
CHEK1

Substrates
Peptide substrate, [KKKVSRSGLYRSPSMPENLNRPR], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 0.3
Ro 31-8220 18.7
GW 5074 5,400
H-89 1,200
Rottlerin >100,000
Scientific Information
O14757 (http://www.uniprot.org/uniprot/O14757)

Screening Location
Malvern, USA

Reference compound IC50 for CHK1
Reference compound IC50 for CHK1

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