CHK2(I157T) Kinase Assay Service

Activity-based CHK2(I157T) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
CHK2 (I157T)

Enzyme
Human CHK2 (I157T)

HGNC
CHEK2 (I157T)

Synonym
CDS1, HuCds1, PP1425, RAD53, bA444G7

Substrates
Peptide substrate, [KKKVSRSGLYRSPSMPENLNRPR], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 11
Ro 31-8220 1,000
GW 5074 2,300
H-89 2,800
Rottlerin 6,200
Scientific Information
O96017 (http://www.uniprot.org/uniprot/O96017)

Screening Location
Malvern, USA

Reference compound IC50 for CHK2 (I157T)
Reference compound IC50 for CHK2 (I157T)

PDF