CK1a1 Kinase Assay Service

Activity-based CK1a1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
CK1a1

Enzyme
Human CK1a1

HGNC
CSNK1A1

Synonym
CK1, HLCDGP1, PRO2975, CK1-alpha1

Substrates
Peptide substrate, [KRRRAL[pS]VASLPGL], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 7,800
GW 5074 30,500
Scientific Information
P48729 (http://www.uniprot.org/uniprot/P48729)

Screening Location
Malvern, USA

Reference compound IC50 for CK1a1
Reference compound IC50 for CK1a1

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