Activity-based CK1alpha1 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
CK1alpha1
Enzyme
Human CK1alpha1, wt
HGNC
CSNK1A1
Manning ID
CK1a
Synonym
CK1-alpha1, CK1, HLCDGP1, PRO2975, CSNK1A1, CK1alpha1, CK1a1
UniProt
P48729 (KC1A_HUMAN)
Kinase Type
Protein kinase
Kinase Group
CK1
Assay Properties
Assay Format
33PanQinaseTM
Substrates
Casein, ATP
Reaction
Casein + 33P-ATP = 33P-Casein + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Sorafenib | 10000 |
| Sunitinib | 2600 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | CK1alpha1 |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – CK1alpha1 (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 0406-0000-1 |
| Web Shop | Reaction Biology Online Shop |
PDF