Activity-based CK1epsilon kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
CK1epsilon
Enzyme
Human CK1epsilon
HGNC
CSNK1E
Synonym
CKIe, HCKIE, MGC10398, CK1-epsilon
Substrates
Peptide substrate, [KRRRAL[pS]VASLPGL], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 3,600 |
| Ro 31-8220 | 10,100 |
| GW 5074 | 2,600 |
| H-89 | 66,800 |
| D4476 | 350 |
Scientific Information
P49674 (http://www.uniprot.org/uniprot/P49674)
Screening Location
Malvern, USA
Reference compound IC50 for CK1epsilon
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